Efficient Antibody Assembly in E. coli Periplasm by Disulfide Bond Folding Factor Co-expression and Culture Optimization

Appl Biochem Biotechnol. 2017 Oct;183(2):520-529. doi: 10.1007/s12010-017-2502-8. Epub 2017 May 10.


Molecular chaperones and protein folding factors of bacterial periplasmic space play important roles in assisting disulfide bond formation and proper protein folding. In this study, effects of disulfide bond protein (Dsb) families were investigated on assembly of 3F3 Fab, an antibody inhibitor targeting matrix metalloproteinase-14 (MMP-14). By optimizing DsbA/C co-expression, promoter for 3F3 Fab, host strains, and culture media and conditions, a high yield of 30-mg purified 3F3 Fab per liter culture was achieved. Produced 3F3 Fab exhibited binding affinity of 34 nM and inhibition potency of 970 nM. This established method of DsbA/C co-expression can be applied to produce other important disulfide bond-dependent recombinant proteins in E. coli periplasm.

Keywords: DsbA; DsbC; Fab; IgG; Over-expression; Periplasm; Protein folding factor.

MeSH terms

  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism*
  • Humans
  • Immunoglobulin Fab Fragments / biosynthesis*
  • Immunoglobulin Fab Fragments / genetics
  • Periplasm / genetics
  • Periplasm / metabolism*
  • Protein Disulfide-Isomerases / genetics
  • Protein Disulfide-Isomerases / metabolism*
  • Protein Folding*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics


  • Escherichia coli Proteins
  • Immunoglobulin Fab Fragments
  • Recombinant Proteins
  • Protein Disulfide-Isomerases
  • dsbA protein, E coli
  • dsbC protein, E coli