Tumor suppressive function of microRNA-192 in acute lymphoblastic leukemia

Bosn J Basic Med Sci. 2017 Aug 20;17(3):248-254. doi: 10.17305/bjbms.2017.1921.

Abstract

Non-coding RNAs play a critical role in gene regulation in cancer cells. Reduced expression of microRNA-192 (miR-192) has been detected in many cancers. In this study, we investigated the role of miR-192 in cell proliferation and cell cycle control in NALM-6 cell line, a model of acute lymphoblastic leukemia (ALL). Cell cycle analysis by DNA content using propidium iodide staining and cell apoptosis analysis using Annexin V assay were carried out. Cell proliferation changes were monitored using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. In addition, the relative changes in P53, BAX, CASP3, and BCL-2 gene expression were determined by quantitative reverse transcription PCR. Overexpression of miR-192 resulted in cell proliferation arrest in ALL cells. After 72 and 96 hours of transduction, apoptosis was significantly increased in the cells transduced with miR-192-overexpressing virus compared with control cells. The expression of P53, BAX, and CASP3 increased after 48 hours of transduction in miR-192-overexpressing cells, but no change was observed in BCL-2 expression. The G0/S and G1/S ratio changed to 7.5 and 4.5, respectively, in the cells overexpressing miR-192 compared with controls. The results of our study suggest, for the first time, tumor suppressive effects of miR-192 in ALL cells.

MeSH terms

  • Apoptosis / drug effects
  • Cell Line, Tumor
  • Cell Proliferation
  • G1 Phase / drug effects
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor*
  • Genetic Vectors
  • Humans
  • Lentivirus / genetics
  • MicroRNAs / genetics*
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / genetics
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
  • Resting Phase, Cell Cycle / drug effects
  • Transfection

Substances

  • MIRN192 microRNA, human
  • MicroRNAs
  • Neoplasm Proteins