Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1

Environ Microbiol Rep. 2017 Aug;9(4):404-410. doi: 10.1111/1758-2229.12545. Epub 2017 Jun 6.

Abstract

Pyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functionality of methylamine methyltransferases, an important component of corrinoid-dependent methyltransfer reactions, depends on the presence of Pyl. Here, we present a putative pyl gene cluster obtained from single-cell genomes of the archaeal Mediterranean Sea Brine Lakes group 1 (MSBL1) from the Red Sea. Functional annotation of the MSBL1 single cell amplified genomes (SAGs) also revealed a complete corrinoid-dependent methyl-transfer pathway suggesting that members of MSBL1 may possibly be capable of synthesizing Pyl and metabolizing methylated amines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Archaea / genetics*
  • Archaea / growth & development
  • Archaea / isolation & purification
  • Archaea / metabolism*
  • Archaeal Proteins / genetics
  • Archaeal Proteins / metabolism
  • Codon, Terminator / genetics
  • Genome, Archaeal
  • Genomics
  • Indian Ocean
  • Lysine / analogs & derivatives*
  • Lysine / biosynthesis
  • Methyltransferases / genetics
  • Methyltransferases / metabolism
  • Phylogeny

Substances

  • Archaeal Proteins
  • Codon, Terminator
  • Methyltransferases
  • pyrrolysine
  • Lysine