Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma

Eur J Immunol. 1988 Nov;18(11):1819-26. doi: 10.1002/eji.1830181125.


Using a new Ly-6C-specific antibody (Monts-1) we show that this class of antigens are differentially expressed on monocytes/macrophages and endothelial cells. Recently elicited peritoneal exudate Mac-1+ mononuclear cells, as well as Mac-1+ mononuclear cells in the bone marrow and in the peripheral blood, express high levels of Ly-6C. Ly-6C+ mononuclear Mac-1+ cells are absent in normal uninflamed skin, but are present in high numbers in skin lesions 3 days after the s.c. injection of lipopolysaccharide, concanavalin A or complete Freund's adjuvant. In addition, large Ly-6C+ mononuclear cells are predominant in chronic granulomas induced by complete Freund's adjuvant. Resident macrophages in a variety of tissues express low levels or in many cases do not express Ly-6C. Two out of three monocyte-like cell lines are Ly-6C+, whereas macrophage-like cell lines are negative. Ly-6C+ monocytes/macrophages lose the Ly-6C antigen within 24 h after in vitro culture. Ly-6C- cultured monocytes and Ly-6C- monocyte-like cell lines, but not fully differentiated macrophages and macrophage-like cell lines, can be induced to express the Ly-6C antigen by interferon-gamma. A population of small vessel endothelial cells in diverse tissues also express high levels of Ly-6C. The present findings suggest that the Ly-6C antigen family, shown by others to be involved in T cell activation, may have more general importance in immune responses and cellular differentiation than previously appreciated.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigens, Differentiation, Myelomonocytic / immunology*
  • Antigens, Surface / immunology*
  • Blotting, Western
  • Cell Line
  • Endothelium / immunology*
  • Flow Cytometry
  • Immunohistochemistry
  • Interferon-gamma / pharmacology*
  • Macrophages / classification
  • Macrophages / immunology*
  • Mice
  • Molecular Weight
  • Monocytes / classification
  • Monocytes / immunology*
  • Tumor Necrosis Factor Receptor Superfamily, Member 7


  • Antibodies, Monoclonal
  • Antigens, Differentiation, Myelomonocytic
  • Antigens, Surface
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Interferon-gamma