Analysis of an RNA-seq Strand-Specific Library from an East Timorese Cucumber Sample Reveals a Complete Cucurbit aphid-borne yellows virus Genome

Solomon Maina; Owain R Edwards; Luis de Almeida; Abel Ximenes; Roger A C Jones

doi:10.1128/genomeA.00320-17

Analysis of an RNA-seq Strand-Specific Library from an East Timorese Cucumber Sample Reveals a Complete Cucurbit aphid-borne yellows virus Genome

Genome Announc. 2017 May 11;5(19):e00320-17. doi: 10.1128/genomeA.00320-17.

Authors

Solomon Maina^{1

2

3}, Owain R Edwards^{3

4}, Luis de Almeida⁵, Abel Ximenes⁶, Roger A C Jones^{7

3

8}

Affiliations

¹ School of Agriculture and Environment, Faculty of Science, University of Western Australia, Crawley, Western Australia, Australia.
² Institute of Agriculture, Faculty of Science, University of Western Australia, Crawley, Western Australia, Australia.
³ Cooperative Research Centre for Plant Biosecurity, Canberra, Australian Capital Territory, Australia.
⁴ CSIRO Land and Water, Floreat Park, Western Australia, Australia.
⁵ Seeds of Life Project, Ministry of Agriculture, Forestry and Fisheries, Dili, East Timor.
⁶ DNQB-Plant Quarantine, International Airport Nicolau Lobato Comoro, Dili, East Timor.
⁷ Institute of Agriculture, Faculty of Science, University of Western Australia, Crawley, Western Australia, Australia roger.jones@uwa.edu.au.
⁸ Department of Agriculture and Food Western Australia, South Perth, Western Australia, Australia.

Abstract

Analysis of an RNA-seq library from cucumber leaf RNA extracted from a fast technology for analysis of nucleic acids (FTA) card revealed the first complete genome of Cucurbit aphid-borne yellows virus (CABYV) from East Timor. We compare it with 35 complete CABYV genomes from other world regions. It most resembled the genome of the South Korean isolate HD118.