Evidence for a nuclear factor(s), IRF-1, mediating induction and silencing properties to human IFN-beta gene regulatory elements

EMBO J. 1988 Nov;7(11):3397-405.

Abstract

Transcription of the human interferon-beta (IFN-beta) gene is induced by a variety of agents such as viruses, dsRNA and some cytokines. In this study, we describe a nuclear factor, termed interferon regulatory factor-1 (IRF-1), that is involved in the transcription of IFN-beta and possibly other genes. We demonstrate that IRF-1 functions in virus-induced transcription by interacting with previously identified, IFN-beta regulatory DNA elements. Our data suggest that IRF-1 participates in the transient formation of an induction-specific complex(es) with the regulatory elements. IRF-1 may also be involved in silencing the function of the SV40 enhancer juxtaposed to the regulatory elements in uninduced cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • DNA / genetics
  • DNA / metabolism
  • DNA Probes
  • Enhancer Elements, Genetic
  • Gene Expression Regulation*
  • Genes, Regulator*
  • Humans
  • Interferon Type I / genetics*
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Plasmids
  • Restriction Mapping
  • Simian virus 40 / genetics
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcription, Genetic*
  • Transfection

Substances

  • DNA Probes
  • Interferon Type I
  • Nuclear Proteins
  • Transcription Factors
  • DNA