Nm-seq maps 2'-O-methylation sites in human mRNA with base precision

Nat Methods. 2017 Jul;14(7):695-698. doi: 10.1038/nmeth.4294. Epub 2017 May 15.


The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.

MeSH terms

  • Base Sequence
  • HeLa Cells
  • Humans
  • Metagenomics
  • Methylation
  • Nucleic Acid Conformation
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics*
  • RNA, Ribosomal / chemistry
  • RNA, Ribosomal / genetics
  • Ribose / chemistry
  • Transcriptome


  • RNA, Messenger
  • RNA, Ribosomal
  • Ribose