Graphene Oxide Conjugated Magnetic Beads for RNA Extraction

Chem Asian J. 2017 Aug 4;12(15):1883-1888. doi: 10.1002/asia.201700554. Epub 2017 Jun 22.


A magnetic material that consists of silica-coated magnetic beads conjugated with graphene oxide (GO) was successfully prepared for facile ribonucleic acid (RNA) extraction. When the GO-modified magnetic beads were applied to separate the RNA from the lysed cell, the cellular RNAs were readily adsorbed to and readily desorbed from the surface of the GO-modified magnetic beads by urea. The amount of RNA extracted by the GO-modified magnetic beads was ≈170 % as much as those of the control extracted by a conventional phenol-based chaotropic solution. These results demonstrate that the facile method of RNA separation by using GO-modified magnetic beads as an adsorbent is an efficient and simple way to purify intact cellular RNAs and/or microRNA from cell lysates.

Keywords: RNA extraction; RNA separation; graphene oxide; magnetic beads; silica coating.

MeSH terms

  • Chemistry Techniques, Analytical / methods*
  • Electrophoresis, Gel, Two-Dimensional
  • Graphite / chemistry*
  • Magnetics*
  • Microscopy, Electron, Scanning
  • Nanoparticles / chemistry
  • RNA / chemistry
  • RNA / isolation & purification*
  • Time Factors


  • RNA
  • Graphite