Effects of Vaccination with the C-Strain Vaccine on Immune Cells and Cytokines of Pigs Against Classical Swine Fever Virus

Viral Immunol. 2018 Jan/Feb;31(1):34-39. doi: 10.1089/vim.2017.0010. Epub 2017 May 17.


The attenuated C-strain vaccine against classical swine fever virus (CSFV) is one of the safest and most effective attenuated vaccines. However, little is known of the host immune response after vaccination with the C-strain vaccine. Blood samples from vaccinated pigs were collected to evaluate the number of immune cells, the level of specific CSFV antibody, and related cytokines induced by the vaccination of C-strain vaccine. The C-strain nucleic acid was gradually removed and specific antibody to vaccine kept increasing; the amount of the lymphocyte, Tc cell, and Th cell increased; some inflammatory cytokines such as interleukin (IL)-1 and tumor necrosis factor-α mainly showed downregulated trends, but IL-6 and IL-8 were upregulated greatly; IL-2, IL-4, IL-5, IL-12p40, IL-13, interferon (IFN)-I, and Toll-like receptors (TLRs) kept high expression level after 28 days postvaccination (dpv); IFN-γ was upregulated slightly at 5 and 9 dpv, respectively. These results suggest that the C-strain vaccine induces a Th2 cell response to produce the specific antibody. The vaccine virus replicates at very low level. C-strain vaccine burden has close relationship with the expression of TLRs. The overexpression of TLRs initiates the innate immune system to clear up the vaccine. Meanwhile, ILs expressed by immune system induce the differentiation of B cells and produce specific antibody.

Keywords: C-strain vaccine; classical swine fever; cytokine; immune cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Classical Swine Fever / blood
  • Classical Swine Fever / immunology
  • Classical Swine Fever / prevention & control*
  • Classical Swine Fever Virus / immunology*
  • Cytokines / genetics*
  • Gene Expression Regulation
  • Kinetics
  • Lymphocyte Count
  • Male
  • RNA, Viral / analysis
  • Swine / immunology*
  • Swine / virology
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocytes, Cytotoxic / immunology
  • T-Lymphocytes, Helper-Inducer / immunology
  • Toll-Like Receptors / genetics
  • Vaccination / veterinary*
  • Vaccines, Attenuated / administration & dosage
  • Vaccines, Attenuated / immunology
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / immunology*


  • Antibodies, Viral
  • Cytokines
  • RNA, Viral
  • Toll-Like Receptors
  • Vaccines, Attenuated
  • Viral Vaccines