Evolutionary enhancement of Zika virus infectivity in Aedes aegypti mosquitoes

Nature. 2017 May 25;545(7655):482-486. doi: 10.1038/nature22365. Epub 2017 May 17.


Zika virus (ZIKV) remained obscure until the recent explosive outbreaks in French Polynesia (2013-2014) and South America (2015-2016). Phylogenetic studies have shown that ZIKV has evolved into African and Asian lineages. The Asian lineage of ZIKV was responsible for the recent epidemics in the Americas. However, the underlying mechanisms through which ZIKV rapidly and explosively spread from Asia to the Americas are unclear. Non-structural protein 1 (NS1) facilitates flavivirus acquisition by mosquitoes from an infected mammalian host and subsequently enhances viral prevalence in mosquitoes. Here we show that NS1 antigenaemia determines ZIKV infectivity in its mosquito vector Aedes aegypti, which acquires ZIKV via a blood meal. Clinical isolates from the most recent outbreak in the Americas were much more infectious in mosquitoes than the FSS13025 strain, which was isolated in Cambodia in 2010. Further analyses showed that these epidemic strains have higher NS1 antigenaemia than the FSS13025 strain because of an alanine-to-valine amino acid substitution at residue 188 in NS1. ZIKV infectivity was enhanced by this amino acid substitution in the ZIKV FSS13025 strain in mosquitoes that acquired ZIKV from a viraemic C57BL/6 mouse deficient in type I and II interferon (IFN) receptors (AG6 mouse). Our results reveal that ZIKV evolved to acquire a spontaneous mutation in its NS1 protein, resulting in increased NS1 antigenaemia. Enhancement of NS1 antigenaemia in infected hosts promotes ZIKV infectivity and prevalence in mosquitoes, which could have facilitated transmission during recent ZIKV epidemics.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes / virology*
  • Americas / epidemiology
  • Amino Acid Sequence
  • Amino Acid Substitution
  • Animals
  • Asia / epidemiology
  • Biological Evolution*
  • Cambodia / epidemiology
  • Female
  • Humans
  • Life Cycle Stages
  • Mice
  • Mice, Inbred C57BL
  • Mosquito Vectors / virology*
  • Phylogeny
  • Vero Cells
  • Viral Nonstructural Proteins / chemistry
  • Viral Nonstructural Proteins / genetics
  • Viral Nonstructural Proteins / metabolism
  • Zika Virus / genetics
  • Zika Virus / isolation & purification
  • Zika Virus / metabolism
  • Zika Virus / pathogenicity*
  • Zika Virus Infection / epidemiology
  • Zika Virus Infection / transmission*
  • Zika Virus Infection / virology*


  • Viral Nonstructural Proteins