Operon structure of flagellar genes in Salmonella typhimurium

Mol Gen Genet. 1988 Sep;214(1):11-5. doi: 10.1007/BF00340172.

Abstract

In Salmonella typhimurium, more than 40 genes have been shown to be involved in flagellar formation and function and almost all of them have been assigned to three regions of the chromosome, termed region I, region II, and region III. In the present study, a large number of transposon-insertion mutants in these flagellar genes were isolated using Tn10 and Mud1. The flaV gene was found to be a strong hot spot for Tn10 insertion. Complementation analysis of the polarity effects exerted by the transposon-insertion mutants defined 13 different flagellar operons; 3 in region I, 4 in region II, and 6 in region III. These results are compared with the reported arrangement of the corresponding genes in Escherichia coli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Transposable Elements
  • DNA, Bacterial / ultrastructure
  • Flagella / ultrastructure*
  • Gene Expression
  • Genes, Bacterial*
  • Genetic Complementation Test
  • Mutation
  • Operon*
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / ultrastructure
  • Transduction, Genetic

Substances

  • DNA Transposable Elements
  • DNA, Bacterial