Fresh Frozen Versus Formalin-Fixed Paraffin Embedded for Mass Spectrometry Imaging

Ekta Patel

doi:10.1007/978-1-4939-7051-3_2

Fresh Frozen Versus Formalin-Fixed Paraffin Embedded for Mass Spectrometry Imaging

Methods Mol Biol. 2017:1618:7-14. doi: 10.1007/978-1-4939-7051-3_2.

Author

Ekta Patel¹

Affiliation

¹ Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, City Campus, Howard Street, Sheffield, S1 1WB, UK. epatel1503@gmail.com.

PMID: 28523495
DOI: 10.1007/978-1-4939-7051-3_2

Abstract

Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry Imaging (MSI) is fast becoming an industry leading technique as a means of investigating the distributions of protein and peptide molecules directly from sections of tissue. Developing protocols for the analysis of FFPE tissue opens up numerous opportunities for novel biomarker discovery, due to the large number of tissue banks containing FFPE biopsy samples. This chapter reports the analysis of both fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues using such protocols.

Keywords: Formalin-fixed paraffin embedded (FFPE); Fresh frozen; MALDI-MSI; Peptides; Trypsin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Formaldehyde / chemistry*
Humans
Paraffin Embedding / methods*
Peptides / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Tissue Fixation / methods*

Substances

Peptides
Formaldehyde