DESI Mass Spectrometry Imaging (MSI)

Emmanuelle Claude; Emrys A Jones; Steven D Pringle

doi:10.1007/978-1-4939-7051-3_7

DESI Mass Spectrometry Imaging (MSI)

Methods Mol Biol. 2017:1618:65-75. doi: 10.1007/978-1-4939-7051-3_7.

Authors

Emmanuelle Claude¹, Emrys A Jones², Steven D Pringle²

Affiliations

¹ Waters Corporation, Stamford Avenue, Altrincham Road, Wilmslow, SK9 4AX, UK. emmanuelle_claude@waters.com.
² Waters Corporation, Stamford Avenue, Altrincham Road, Wilmslow, SK9 4AX, UK.

PMID: 28523500
DOI: 10.1007/978-1-4939-7051-3_7

Abstract

Desorption Electrospray Ionization (DESI) mass spectrometry is a technique that allows chemical information to be obtained directly from a wide range of surfaces. Using a 2D stage, DESI can be implemented in an imaging mode whereby MS spectra are collected by rastering the spray across the whole surface. Here, we describe the implementation and optimization of DESI imaging for metabolites and lipids from tissue sections using oa-TOF mass spectrometers.

Keywords: DESI; Imaging; Lipids; MS; MSI.

MeSH terms

Lipids / chemistry*
Mass Spectrometry / methods
Spectrometry, Mass, Electrospray Ionization / methods*

Substances

Lipids