"Expand and Click": A New Method for Labeling HIV-1 Envelope Glycoproteins

Melissa V Fernandez; Eric O Freed

doi:10.1016/j.chembiol.2017.05.006

"Expand and Click": A New Method for Labeling HIV-1 Envelope Glycoproteins

Cell Chem Biol. 2017 May 18;24(5):548-550. doi: 10.1016/j.chembiol.2017.05.006.

Authors

Melissa V Fernandez¹, Eric O Freed²

Affiliations

¹ Virus-Cell Interaction Section, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702-1201, USA.
² Virus-Cell Interaction Section, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702-1201, USA. Electronic address: efreed@nih.gov.

Abstract

In this issue of Cell Chemical Biology, Sakin et al. (2017) investigate the nanoscale behavior of the HIV-1 envelope (Env) glycoprotein complex by using genetic code expansion, bioorthogonal amino acids, synthetic dyes, and click chemistry. This minimally invasive approach allows the measurement of native Env cellular distribution and dynamics.

Published by Elsevier Ltd.

MeSH terms

Binding Sites
Click Chemistry
HIV-1*
Staining and Labeling / methods*
env Gene Products, Human Immunodeficiency Virus / chemistry*
env Gene Products, Human Immunodeficiency Virus / metabolism*

Substances

env Gene Products, Human Immunodeficiency Virus

Grants and funding

ZIA BC011721/ImNIH/Intramural NIH HHS/United States