Oxygenation and adenosine deaminase support growth and proliferation of ex vivo cultured Drosophila wing imaginal discs

Development. 2017 Jul 1;144(13):2529-2538. doi: 10.1242/dev.147538. Epub 2017 May 19.


The Drosophila wing imaginal disc has been an important model system over past decades for discovering novel biology related to development, signaling and epithelial morphogenesis. Novel experimental approaches have been enabled using a culture setup that allows ex vivo cultures of wing discs. Current setups, however, are not able to sustain both growth and cell-cycle progression of wing discs ex vivo We discover here a setup that requires both oxygenation of the tissue and adenosine deaminase activity in the medium, and supports both growth and proliferation of wing discs for 9 h. Nonetheless, further work will be required to extend the duration of the culturing and to enable live imaging of the cultured discs in the future.

Keywords: Adenosine deaminase; Culture; Development; Drosophila; Oxygenation; Wing discs.

MeSH terms

  • Adenosine Deaminase / metabolism*
  • Animals
  • Cell Proliferation
  • Cells, Cultured
  • Drosophila melanogaster / cytology*
  • Drosophila melanogaster / enzymology*
  • Ecdysone / metabolism
  • Ethidium / metabolism
  • Fat Body / cytology
  • Fat Body / metabolism
  • Imaginal Discs / cytology*
  • Insulin / metabolism
  • Juvenile Hormones / metabolism
  • Oxygen / metabolism*
  • S Phase
  • Wings, Animal / cytology*


  • Insulin
  • Juvenile Hormones
  • Ecdysone
  • Adenosine Deaminase
  • Ethidium
  • Oxygen