Single-step PCR-based genetic sex determination of rat tissues and cells

Biotechniques. 2017 May 1;62(5):232-233. doi: 10.2144/000114548.

Abstract

The advent of genome editing strategies has expanded the range of animal models available for gene manipulation and renewed research interest in the rat. Gender is a key variable for in vivo gene function analyses. Here, we present a simple PCR-based method to determine genetic sex in the rat.

Keywords: fetal; rat; sex determination.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Female
  • Male
  • Polymerase Chain Reaction / methods*
  • Rats
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Sequence Analysis, DNA / methods*
  • Sex Chromosomes / genetics*
  • Sex Determination Analysis / methods*