Evaluation of plasma sphingosine 1-phosphate, hepcidin and cardiovascular damage biomarkers (cardiac troponin I and homocysteine) in rats infected with brucellosis and vaccinated (Rev-1, RB-51)

Microb Pathog. 2017 Aug:109:67-70. doi: 10.1016/j.micpath.2017.05.031. Epub 2017 May 19.


Brucellosis is known as one of important zoonosis. Studying the histological and biochemical effects of the disease could help to increase our knowledge about it. The aim of the present study was to evaluate changes of plasma parameters after intraperitoneal injection of two species of Brucella (Brucella melitensis and Brucella abortus) and two vaccines (Rev-1, RB-51) in the rat. Forty male rats were divided into five groups (n = 8 in each group). Two groups received suspensions of Brucella abortus and Brucella melitensis and two other groups were injected intraperitoneally with two mentioned vaccines and the last group received only distilled water. The results showed a significant increase in sphingosine 1-phosphate, Malondialdehyde, hepcidin, homocysteine, cardiac troponin I and copper levels and a considerable decrease in the levels of iron and zinc (P ≤ 0.01) in infected groups compared to the control animals. In vaccinated groups, hepcidin was increased but other parameters were not changed in comparison to the control group. It can be concluded that increase of homocysteine and cardiac troponin I in brucellosis could be a warning for cardiac adverse effects. Besides, increase of sphingosine 1-phosphate probably indicates its stimulant and modulatory effects in anti- Brucellosis biochemical pathways of the host.

Keywords: Brucellosis; Cardiac troponin I; Hepcidin; Homocysteine; Sphingosine 1-phosphate.

MeSH terms

  • Animals
  • Biomarkers / blood*
  • Blood Chemical Analysis
  • Brucella Vaccine / immunology*
  • Brucella abortus / immunology
  • Brucella abortus / pathogenicity
  • Brucella melitensis / immunology
  • Brucella melitensis / pathogenicity
  • Brucellosis / immunology*
  • Brucellosis / prevention & control
  • Copper / blood
  • Disease Models, Animal
  • Hepcidins / blood*
  • Homocysteine / blood*
  • Iron / blood
  • Lysophospholipids / blood*
  • Male
  • Malondialdehyde / blood
  • Plasma / chemistry*
  • Rats
  • Sphingosine / analogs & derivatives*
  • Sphingosine / blood
  • Troponin I / blood*
  • Vaccination
  • Zinc / blood


  • Biomarkers
  • Brucella Vaccine
  • Hepcidins
  • Lysophospholipids
  • Troponin I
  • Homocysteine
  • sphingosine 1-phosphate
  • Malondialdehyde
  • Copper
  • Iron
  • Zinc
  • Sphingosine