Tn916 Delta E: A Tn916 Transposon Derivative Expressing Erythromycin Resistance

Plasmid. 1988 Sep;20(2):137-42. doi: 10.1016/0147-619x(88)90016-9.

Abstract

The tetracycline resistance gene encoded within the transposon Tn916 was replaced with the gene encoding erythromycin resistance from the plasmid pVA838. The derivative transposon of Tn916 was designated Tn916 delta E and was introduced into the Streptococcus faecalis chromosome by protoplast transformation. The conjugation/transposition functions of Tn916 delta E were similar to those observed for Tn916 in S. faecalis and Tn916 delta E was capable of self-conjugation at frequencies similar to those of other S. faecalis and Group B Streptococcus. This transposon will be useful for mutagenesis studies in gram-positive organisms, especially in those species where erythromycin resistance is a more desirable selectable marker.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Southern
  • Conjugation, Genetic
  • DNA Transposable Elements*
  • Drug Resistance, Microbial / genetics
  • Enterococcus faecalis / drug effects
  • Enterococcus faecalis / genetics*
  • Erythromycin / pharmacology*
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Genes, Bacterial
  • Genetic Vectors
  • Nucleic Acid Hybridization
  • R Factors*

Substances

  • DNA Transposable Elements
  • Erythromycin