Tn916 Delta E: A Tn916 Transposon Derivative Expressing Erythromycin Resistance

Plasmid. 1988 Sep;20(2):137-42. doi: 10.1016/0147-619x(88)90016-9.


The tetracycline resistance gene encoded within the transposon Tn916 was replaced with the gene encoding erythromycin resistance from the plasmid pVA838. The derivative transposon of Tn916 was designated Tn916 delta E and was introduced into the Streptococcus faecalis chromosome by protoplast transformation. The conjugation/transposition functions of Tn916 delta E were similar to those observed for Tn916 in S. faecalis and Tn916 delta E was capable of self-conjugation at frequencies similar to those of other S. faecalis and Group B Streptococcus. This transposon will be useful for mutagenesis studies in gram-positive organisms, especially in those species where erythromycin resistance is a more desirable selectable marker.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Southern
  • Conjugation, Genetic
  • DNA Transposable Elements*
  • Drug Resistance, Microbial / genetics
  • Enterococcus faecalis / drug effects
  • Enterococcus faecalis / genetics*
  • Erythromycin / pharmacology*
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Genes, Bacterial
  • Genetic Vectors
  • Nucleic Acid Hybridization
  • R Factors*


  • DNA Transposable Elements
  • Erythromycin