Cloning and functional characterization of a retinoid X receptor orthologue in Platynereis dumerilii: An evolutionary and toxicological perspective

Ana André; Raquel Ruivo; Ana Capitão; Elsa Froufe; Inês Páscoa; Luís Filipe Costa Castro; Miguel Machado Santos

doi:10.1016/j.chemosphere.2017.05.064

Cloning and functional characterization of a retinoid X receptor orthologue in Platynereis dumerilii: An evolutionary and toxicological perspective

Chemosphere. 2017 Sep:182:753-761. doi: 10.1016/j.chemosphere.2017.05.064. Epub 2017 May 20.

Authors

Ana André¹, Raquel Ruivo², Ana Capitão³, Elsa Froufe⁴, Inês Páscoa⁵, Luís Filipe Costa Castro⁶, Miguel Machado Santos⁷

Affiliations

¹ CIMAR/CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Avenida General Norton de Matos s/n, 4450-208, Matosinhos, Portugal; ICBAS - Institute of Biomedical Sciences Abel Salazar, University of Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313, Porto, Portugal. Electronic address: iandre@ciimar.up.pt.
² CIMAR/CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Avenida General Norton de Matos s/n, 4450-208, Matosinhos, Portugal. Electronic address: rruivo@ciimar.up.pt.
³ CIMAR/CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Avenida General Norton de Matos s/n, 4450-208, Matosinhos, Portugal; FCUP-Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007, Porto, Portugal.
⁴ CIMAR/CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Avenida General Norton de Matos s/n, 4450-208, Matosinhos, Portugal. Electronic address: efonseca@ciimar.up.pt.
⁵ CIMAR/CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Avenida General Norton de Matos s/n, 4450-208, Matosinhos, Portugal.
⁶ CIMAR/CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Avenida General Norton de Matos s/n, 4450-208, Matosinhos, Portugal; FCUP-Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007, Porto, Portugal. Electronic address: filipe.castro@ciimar.up.pt.
⁷ CIMAR/CIIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Avenida General Norton de Matos s/n, 4450-208, Matosinhos, Portugal; FCUP-Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007, Porto, Portugal. Electronic address: santos@ciimar.up.pt.

PMID: 28535483
DOI: 10.1016/j.chemosphere.2017.05.064

Abstract

In the present work we provide the first isolation and functional characterization of a RXR orthologue in an annelid species, the Platynereis dumerilii. Using an in vitro luciferase reporter assay we evaluated the annelid receptor ability to respond to ligand 9-cis-retinoic acid, TBT and TPT. Our results show that the annelid RXR responds to 9-cis-retinoic acid and to the organotins by activating reporter gene transcription. The findings suggest a conserved mode of action of the receptor in response to a common signaling molecule and modulation by organotin compounds between vertebrates and Lophotrochozoans.

Keywords: Annelid; Endocrine disruption; Evolution; Retinoid X receptor; Tributyltin; Triphenyltin.

MeSH terms

Alitretinoin
Animals
Biological Evolution
Gene Expression Regulation / physiology
Ligands
Organotin Compounds
Polychaeta / metabolism*
Retinoid X Receptors / genetics
Retinoid X Receptors / metabolism*
Transcriptional Activation / drug effects
Tretinoin / metabolism

Substances

Ligands
Organotin Compounds
Retinoid X Receptors
Alitretinoin
Tretinoin