The identification of human aldo-keto reductase AKR7A2 as a novel cytoglobin-binding partner

Cell Mol Biol Lett. 2016 Oct 24;21:25. doi: 10.1186/s11658-016-0026-9. eCollection 2016.


Cytoglobin (CYGB), a member of the globin family, is thought to protect cells from reactive oxygen and nitrogen species and deal with hypoxic conditions and oxidative stress. However, its molecular mechanisms of action are not clearly understood. Through immunoprecipitation combined with a two-dimensional electrophoresis-mass spectrometry assay, we identified a CYGB interactor: aldo-keto reductase family 7 member A2 (AKR7A2). The interaction was further confirmed using yeast two-hybrid and co-immunoprecipitation assays. Our results show that AKR7A2 physically interacts with CYGB.

Keywords: 2-DE; AKR7A2; CYGB; Co-immunoprecipitation; Oxidative stress; Protein-protein interactions; Yeast two-hybrid assay.

MeSH terms

  • Aldehyde Reductase / metabolism*
  • Cell Line
  • Cytoglobin
  • Globins / metabolism*
  • HEK293 Cells
  • Humans
  • Immunoprecipitation
  • Mass Spectrometry
  • Protein Binding
  • Two-Hybrid System Techniques


  • CYGB protein, human
  • Cytoglobin
  • Globins
  • aflatoxin B1 aldehyde reductase
  • Aldehyde Reductase