Stress-Induced Transcriptional Changes and DNA Damage Associated with Bis(2-ethylhexyl) Adipate Exposure in Zebrafish (Danio rerio) Larvae

Halis Boran; Serap Terzi

doi:10.1007/s00128-017-2116-4

Stress-Induced Transcriptional Changes and DNA Damage Associated with Bis(2-ethylhexyl) Adipate Exposure in Zebrafish (Danio rerio) Larvae

Bull Environ Contam Toxicol. 2017 Sep;99(3):308-314. doi: 10.1007/s00128-017-2116-4. Epub 2017 May 24.

Authors

Halis Boran¹, Serap Terzi²

Affiliations

¹ Faculty of Fisheries, Recep Tayyip Erdoğan University, 53100, Rize, Turkey. halisboran@gmail.com.
² Faculty of Fisheries, Recep Tayyip Erdoğan University, 53100, Rize, Turkey.

PMID: 28536799
DOI: 10.1007/s00128-017-2116-4

Abstract

The present study evaluates potential toxic effects of bis(2-ethylhexyl) adipate (DEHA) plasticizer to larval (72 h post fertilization) zebrafish (Danio rerio) by analyzing changes in expression levels of stress-related genes (p53, rad51 and xrcc5) and assessing possible DNA damage of DEHA in larvae. The lethal concentration for 50% mortality (LC₅₀) in larval zebrafish exposed for 96 h to 0-200 mg L^-1 DEHA was 89.9 ± 8.03 mg L^-1. A concentration-dependent increase in DNA strand breaks was detected in cells from larvae exposed for 96 h to DEHA. There were some significant differences in induction of stress-related genes in larvae exposed to DEHA relative to control.

Keywords: Acute toxicity; Bis(2-ethylhexyl) adipate; Gene expression; Genotoxicity; Zebrafish.

MeSH terms

Adipates / toxicity*
Animals
DNA Damage / drug effects*
Gene Expression Regulation, Developmental / drug effects*
Larva / drug effects
Plasticizers
Water Pollutants, Chemical / toxicity*
Zebrafish / metabolism

Substances

Adipates
Plasticizers
Water Pollutants, Chemical
dioctyl adipate