TnpA product encoded by the transposable element En-1 of Zea mays is a DNA binding protein

EMBO J. 1988 Dec 20;7(13):4045-53.

Abstract

TnpA protein, the function encoded by the most abundant transcript of En-1 was expressed in Escherichia coli. DNA binding experiments with partially purified tnpA protein revealed that it binds to the subterminal repetitive region of En-1. TnpA protein recognizes a 12-bp-long sequence motif which is reiterated several times at the termini of En-1. Binding is reduced if the cytosine residues of CG dinucleotides and CNG trinucleotides within the motif are methylated. These data suggest a model in which the product of tnpA serves as a regulator of element activity.

MeSH terms

  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • DNA / metabolism
  • DNA Transposable Elements*
  • DNA-Binding Proteins / genetics*
  • Plant Proteins / genetics
  • Plants / genetics*
  • Zea mays / genetics

Substances

  • DNA Transposable Elements
  • DNA-Binding Proteins
  • Plant Proteins
  • DNA