Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma

Gene. 1988 Dec 20;73(2):509-16. doi: 10.1016/0378-1119(88)90515-x.


Two overlapping clones, covering the entire coding sequence of human M2-type pyruvate kinase (PK) cDNA, were isolated and sequenced. Nucleotide sequencing results showed that they contained the 109-bp 5'-untranslated region, the 1593-bp coding region and the 585-bp 3'-untranslated region. Nucleotide sequence homology was 90% and 69% with rat M2-type and L-type PK cDNA, respectively. In situ hybridization using the human M2-type PK cDNA probe disclosed that the gene for M2-type PK is located at band q22 on chromosome 15. Northern blot analysis with RNA from human hepatoma demonstrated that M2-type PK was predominantly expressed in hepatoma cells, whereas L-type PK was preferentially expressed in the non-tumor portion of the liver.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Carcinoma, Hepatocellular / enzymology
  • Carcinoma, Hepatocellular / genetics*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 15*
  • Cloning, Molecular
  • DNA, Recombinant / isolation & purification
  • Genes*
  • Humans
  • Isoenzymes / genetics*
  • Liver Neoplasms / enzymology
  • Liver Neoplasms / genetics*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Pyruvate Kinase / genetics*
  • Restriction Mapping


  • DNA, Recombinant
  • Isoenzymes
  • Pyruvate Kinase

Associated data

  • GENBANK/M23725