Quantitative proteomic analysis revealed changes in protein synthesis and mitochondrial functions after acute DNA damage in mouse neural stem cells

Neurosci Lett. 2017 Jul 13:653:355-361. doi: 10.1016/j.neulet.2017.05.044. Epub 2017 May 25.

Abstract

Considering the accumulation of DNA damages are frequently associated with neurodevelopmental disease, neurodegeneration, and brain tumors, exploration of the molecular mechanisms in mouse neural stem cells (NSCs) after DNA damage would be paramount useful for understanding the pathogenesis of these diseases. In present study, we utilized hydroxyurea (HU) treatment to cultured mouse NSCs to induce acute DNA damages. After HU treatment, mouse NSCs displayed elevated reactive oxygen species (ROS) level and compromised DNA repair in HR and NHEJ pathways. Furthermore, we performed quantitative proteomic analysis to unravel the protein variations. GO analysis and IPA suggested proteins participated in protein synthesis, mitochondrial metabolism and oxidative phosphorylation were under great changes after acute DNA damage. Overall, these data provide valuable insight into the molecular and biological changes in NSCs in the circumstance of acute DNA damage, and will help to discover the connections between DNA damage and potential diseases in brain.

Keywords: DNA damage; Neural stem cells; Neurodegenerative disease; Proteome.

MeSH terms

  • Animals
  • DNA Damage / physiology*
  • DNA Repair / physiology*
  • Mice
  • Mitochondria / metabolism*
  • Neural Stem Cells / metabolism*
  • Protein Biosynthesis / physiology*
  • Proteome / metabolism*
  • Proteomics / methods
  • Reactive Oxygen Species / metabolism*

Substances

  • Proteome
  • Reactive Oxygen Species