Linking E-cadherin mechanotransduction to cell metabolism through force-mediated activation of AMPK

Nat Cell Biol. 2017 Jun;19(6):724-731. doi: 10.1038/ncb3537. Epub 2017 May 29.

Abstract

The response of cells to mechanical force is a major determinant of cell behaviour and is an energetically costly event. How cells derive energy to resist mechanical force is unknown. Here, we show that application of force to E-cadherin stimulates liver kinase B1 (LKB1) to activate AMP-activated protein kinase (AMPK), a master regulator of energy homeostasis. LKB1 recruits AMPK to the E-cadherin mechanotransduction complex, thereby stimulating actomyosin contractility, glucose uptake and ATP production. The increase in ATP provides energy to reinforce the adhesion complex and actin cytoskeleton so that the cell can resist physiological forces. Together, these findings reveal a paradigm for how mechanotransduction and metabolism are linked and provide a framework for understanding how diseases involving contractile and metabolic disturbances arise.

MeSH terms

  • AMP-Activated Protein Kinases / genetics
  • AMP-Activated Protein Kinases / metabolism*
  • Actin Cytoskeleton / metabolism
  • Actomyosin / metabolism
  • Adenosine Triphosphate / metabolism
  • Animals
  • Antigens, CD
  • Cadherins / metabolism*
  • Dogs
  • Energy Metabolism*
  • Enzyme Activation
  • Glucose / metabolism
  • Homeostasis
  • Humans
  • Madin Darby Canine Kidney Cells
  • Mechanotransduction, Cellular*
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism
  • RNA Interference
  • Stress, Mechanical
  • Transfection

Substances

  • Antigens, CD
  • CDH1 protein, human
  • Cadherins
  • Adenosine Triphosphate
  • Actomyosin
  • STK11 protein, human
  • Protein-Serine-Threonine Kinases
  • AMP-Activated Protein Kinases
  • Glucose