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Identification of Atypical El TorV. cholerae O1 Ogawa Hosting SXT Element in Senegal, Africa

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Identification of Atypical El TorV. cholerae O1 Ogawa Hosting SXT Element in Senegal, Africa

Bissoume Sambe-Ba et al. Front Microbiol.

Abstract

Vibrio cholerae O1 is the causative agent of cholera with classical and El Tor, two well-established biotypes. In last 20 years, hybrid strains of classical and El Tor and variant El Tor which carry classical ctxB have emerged worldwide. In 2004-2005, Senegal experienced major cholera epidemic with a number of cases totalling more than 31719 with approximately 458 fatal outcomes (CFR, 1.44%). In this retrospective study, fifty isolates out of a total of 403 V. cholerae biotype El Tor serovar Ogawa isolates from all areas in Senegal during the 2004-2005 cholera outbreak were randomly selected. Isolates were characterized using phenotypic and genotypic methods. The analysis of antibiotic resistance patterns revealed the predominance of the S-Su-TCY-Tsu phenotype (90% of isolates). The molecular characterization of antibiotic resistance revealed the presence of the SXT element, a self-transmissible chromosomally integrating element in all isolates. Most of V. cholerae isolates had an intact virulence cassette (86%) (ctx, zot, ace genes). All isolates tested gave amplification with primers for classical CT, and 10/50 (20%) of isolates carried classical and El Tor ctxB. The study reveals the presence of atypical V. cholerae O1 El Tor during cholera outbreak in Senegal in 2004-2005.

Keywords: O1 virulence; SXT element; Senegal; Vibrio cholerae; antibioresistance.

Figures

FIGURE 1
FIGURE 1
Origin of isolates (Dakar: 39; Diourbel: 09; Kaolack: 01; Louga: 01).
FIGURE 2
FIGURE 2
Agarose gel electrophoresis of examples of PCR products of Vibrio cholerae O1 isolates using tcpA (classical and El Tor).

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