Fangchinoline suppresses growth and metastasis of melanoma cells by inhibiting the phosphorylation of FAK

Oncol Rep. 2017 Jul;38(1):63-70. doi: 10.3892/or.2017.5678. Epub 2017 May 30.

Abstract

Melanoma is a malignant tumor with high degree of malignancy, metastasis and high mortality. The etiology of melanoma has not been fully elucidated, and there is no effective drug for the complete treatment of melanoma. In recent years, many traditional Chinese herbal medicines have played an important role in clinical treatment and experimental research on cancer. As a natural product, fangchinoline has the characteristics of enhancing immune function, low toxicity and good liver protection features, so it is considered to be a new type of anticancer drug. In the present study, we found that fangchinoline has inhibitory effects on the proliferation and metastasis of A375 and A875 cells in a concentration-dependent manner. Fangchinoline inhibited the proliferation of A375 and A875 cell activity with IC50 values of 12.41 and 16.20 µM. We also found that fangchinoline could significantly reduce the phosphorylation of Focal adhesion kinase (FAK). In summary, we demonstrated that fangchinoline inhibits the proliferation and metastasis of melanoma cells by suppressing FAK and its downstream signaling pathway. More importantly, we provide a novel mechanism that fangchinoline could be an effective candidate for the treatment of melanoma.

Publication types

  • Retracted Publication

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Benzylisoquinolines / pharmacology*
  • Cell Movement / drug effects*
  • Cell Proliferation / drug effects*
  • Focal Adhesion Kinase 1 / antagonists & inhibitors*
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Melanoma / drug therapy*
  • Melanoma / metabolism
  • Melanoma / secondary
  • Phosphorylation / drug effects
  • Signal Transduction
  • Tumor Cells, Cultured

Substances

  • Antineoplastic Agents
  • Benzylisoquinolines
  • fangchinoline
  • Focal Adhesion Kinase 1
  • PTK2 protein, human