[Establishment and characterization of a human cholangiocellular carcinoma cell line]

Hum Cell. 1988 Mar;1(1):92-4.
[Article in Japanese]


A cell line, HuH-28 was established in vitro from a patient with cholangiocellular carcinoma (CCC). This cell line has been in continuous culture over 10 month period with slow growth potential. HuH-28 was composed of spindle-shaped cells as major population besides a small percentage of polygonal-shaped cells. Chromosome number of the cells were distributed near the hypotriploid region on the 3rd passage. HuH-28 cells were not transplantable into nude mice, but secreted some tumor markers including alkaline phosphatase (ALP), gamma glutamyltranspeptidase (GGT), beta 2-microglobulin (BMG), ferritin, elastase-1 and tissue polypeptide antigen (TPA). This HuH-28 cell line will represent a good model for the investigation of carcinogenesis, histogenesis+ and diagnosis of CCC.

Publication types

  • English Abstract

MeSH terms

  • Adenoma, Bile Duct / pathology*
  • Adult
  • Animals
  • Bile Duct Neoplasms / pathology*
  • Biomarkers, Tumor / analysis
  • Female
  • Humans
  • Mice
  • Rats
  • Tumor Cells, Cultured*


  • Biomarkers, Tumor