Nearly complete mitogenome of hairy sawfly, Corynis lateralis (Brullé, 1832) (Hymenoptera: Cimbicidae): rearrangements in the IQM and ARNS1EF gene clusters

Genetica. 2017 Oct;145(4-5):341-350. doi: 10.1007/s10709-017-9969-7. Epub 2017 May 31.

Abstract

The Cimbicidae is a small family of the primitive and relatively less diverse suborder Symphyta (Hymenoptera). Here, nearly complete mitochondrial genome (mitogenome) of hairy sawfly, Corynis lateralis (Hymenoptera: Cimbicidae) was sequenced using next generation sequencing and comparatively analysed with the mitogenome of Trichiosoma anthracinum. The sequenced length of C. lateralis mitogenome was 14,899 bp with an A+T content of 80.60%. All protein coding genes (PCGs) are initiated by ATN codons and all are terminated with TAR or T- stop codon. All tRNA genes preferred usual anticodons. Compared with the inferred insect ancestral mitogenome, two tRNA rearrangements were observed in the IQM and ARNS1EF gene clusters, representing a new event not previously reported in Symphyta. An illicit priming of replication and/or intra/inter-mitochondrial recombination and TDRL seem to be responsible mechanisms for the rearrangement events in these gene clusters. Phylogenetic analyses confirmed the position of Corynis within Cimbicidae and recovered a relationship of Tenthredinoidea + (Cephoidea + Orussoidea) in Symphyta.

Keywords: Gene rearrangement; Illicit priming; Next generation sequencing; Phylogeny; Symphyta; TDRL.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Codon
  • Genes, Insect*
  • Genome, Mitochondrial*
  • Hymenoptera / classification
  • Hymenoptera / genetics*
  • Insect Proteins / genetics
  • Multigene Family*
  • Phylogeny
  • RNA, Ribosomal / genetics
  • RNA, Transfer / genetics
  • Sequence Analysis, DNA

Substances

  • Codon
  • Insect Proteins
  • RNA, Ribosomal
  • RNA, Transfer