Direct 1 O 2 optical excitation: A tool for redox biology

Redox Biol. 2017 Oct;13:39-59. doi: 10.1016/j.redox.2017.05.011. Epub 2017 May 25.


Molecular oxygen (O2) displays very interesting properties. Its first excited state, commonly known as singlet oxygen (1O2), is one of the so-called Reactive Oxygen Species (ROS). It has been implicated in many redox processes in biological systems. For many decades its role has been that of a deleterious chemical species, although very positive clinical applications in the Photodynamic Therapy of cancer (PDT) have been reported. More recently, many ROS, and also 1O2, are in the spotlight because of their role in physiological signaling, like cell proliferation or tissue regeneration. However, there are methodological shortcomings to properly assess the role of 1O2 in redox biology with classical generation procedures. In this review the direct optical excitation of O2 to produce 1O2 will be introduced, in order to present its main advantages and drawbacks for biological studies. This photonic approach can provide with many interesting possibilities to understand and put to use ROS in redox signaling and in the biomedical field.

Keywords: Biophotonics; Low-level laser therapy (LLLT); Photodynamic therapy (PDT); Reactive Oxygen Species (ROS); Redox biology; Singlet oxygen.

Publication types

  • Review

MeSH terms

  • Animals
  • Humans
  • Oxidation-Reduction
  • Photochemotherapy / methods
  • Photons*
  • Reactive Oxygen Species / metabolism
  • Singlet Oxygen / chemistry*
  • Singlet Oxygen / metabolism
  • Singlet Oxygen / radiation effects
  • Singlet Oxygen / therapeutic use


  • Reactive Oxygen Species
  • Singlet Oxygen