Stimulation by somatostatin of dephosphorylation of membrane proteins in pancreatic cancer MIA PaCa-2 cell line

FEBS Lett. 1985 Jan 7;179(2):252-6. doi: 10.1016/0014-5793(85)80529-9.

Abstract

A membrane receptor and a cytosolic receptor for somatostatin were found in a human undifferentiated pancreatic cancer cell line (MIA PaCa-2). Binding of somatostatin to this membrane receptor activates dephosphorylation of a phosphotyrosyl-membrane protein whose phosphorylation was promoted by epidermal growth factor (EGF). Vanadate, a purported inhibitor of dephosphorylation, interferes with the action of somatostatin. These findings suggest a possible biochemical mechanism by which somatostatin may inhibit the growth of human pancreatic cancers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Epidermal Growth Factor / pharmacology
  • Humans
  • Membrane Proteins / metabolism*
  • Pancreatic Neoplasms / metabolism*
  • Phosphoprotein Phosphatases / metabolism
  • Phosphoproteins / metabolism*
  • Phosphorylation
  • Receptors, Cell Surface / drug effects
  • Receptors, Cell Surface / physiology
  • Receptors, Somatostatin
  • Somatostatin / analogs & derivatives
  • Somatostatin / metabolism
  • Somatostatin / pharmacology*
  • Vanadates
  • Vanadium / pharmacology

Substances

  • Membrane Proteins
  • Phosphoproteins
  • Receptors, Cell Surface
  • Receptors, Somatostatin
  • Vanadium
  • Vanadates
  • Somatostatin
  • somatostatin, N-Tyr(1)-
  • Epidermal Growth Factor
  • Phosphoprotein Phosphatases