EVALUATION OF THE CHELATING EFFECT OF METHANOLIC EXTRACT OF CORIANDRUM SATIVUM AND ITS FRACTIONS ON WISTAR RATS POISONED WITH LEAD ACETATE

Afr J Tradit Complement Altern Med. 2017 Jan 13;14(2):92-102. doi: 10.21010/ajtcam.v14i2.11. eCollection 2017.

Abstract

Background: The rate of lead poisoning has decreased in recent years due to increased health control in industries that use this metal. However, it is still a public health problem worldwide. The use of various plants with chelating properties has been a topic of research today. In traditional medicine, it is said that Coriandrum sativum has chelating properties, but there is no scientific evidence to support this fact. The purpose of this research is to evaluate the chelating effect of methanol extract of coriander and its fractions on Wistar rats intoxicated with lead.

Materials and methods: In this research, male Wistar rats were poisoned with 50 mg/kg of lead acetate and treated with 50 mg/kg of methanol extract and its fractions. The extract and its fractions were administered to four treatment groups. Positive and negative controls were established. Hemoglobin, hematocrit and lead concentrations were analyzed; liver was evaluated histologically in control and treatment groups.

Results: The methanol extract of coriander presented a LD50 >1000 mg/dL. The group administered with the methanol extract showed significant difference in the levels of hemoglobin and hematocrit compared to the negative control group. Lead concentration in treatment groups showed a decrease compared to the positive control. Histological evaluation of tissue showed less damage in groups administered with methanolic extract and its fractions compared to the positive control which presented structural alterations.

Conclusion: Coriander extracts protect liver and lower lead concentration in rats intoxicated with lead in contrast to the positive control group.

Keywords: Coriandrum sativum; Lead; chelatin effect; natural products.

MeSH terms

  • Animals
  • Chelating Agents / pharmacology
  • Chelating Agents / therapeutic use*
  • Coriandrum*
  • Hematocrit
  • Hemoglobins / metabolism
  • Lead / blood*
  • Lead Poisoning / blood
  • Lead Poisoning / drug therapy*
  • Lead Poisoning / pathology
  • Liver / drug effects*
  • Liver / pathology
  • Male
  • Organometallic Compounds
  • Phytotherapy*
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Rats, Wistar

Substances

  • Chelating Agents
  • Hemoglobins
  • Organometallic Compounds
  • Plant Extracts
  • Lead
  • lead acetate