Automated Cell Culture Systems and Their Applications to Human Pluripotent Stem Cell Studies

SLAS Technol. 2018 Aug;23(4):315-325. doi: 10.1177/2472630317712220. Epub 2017 Jun 2.

Abstract

Pluripotent stem cells are an extremely powerful tool in modeling human diseases and hold much promise for personalized regenerative or cell replacement therapies. There is an increasing need for reproducible large-scale stem cell and differentiated progeny production, with minimal variation, rendering manual approaches impracticable. Here, we provide an overview of systems currently available for automated stem cell culture, and undertake a review of their capacities, capabilities, and relative limitations. With the merging of modern technology and stem cell biology, an increased demand and implementation of automated platforms for stem cell studies is anticipated.

Keywords: automated stem cell system; automation; differentiation; embryonic stem cells; induced pluripotent stem cells; passaging.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Automation*
  • Cell Culture Techniques / methods*
  • Cell- and Tissue-Based Therapy
  • Drug Evaluation, Preclinical
  • Humans
  • Microfluidics
  • Pluripotent Stem Cells / cytology*