CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations

Nat Methods. 2017 Jul;14(7):710-712. doi: 10.1038/nmeth.4327. Epub 2017 Jun 5.


CRISPR-Cas9-induced DNA damage may have deleterious effects at high-copy-number genomic regions. Here, we use CRISPR base editors to knock out genes by changing single nucleotides to create stop codons. We show that the CRISPR-STOP method is an efficient and less deleterious alternative to wild-type Cas9 for gene-knockout studies. Early stop codons can be introduced in ∼17,000 human genes. CRISPR-STOP-mediated targeted screening demonstrates comparable efficiency to WT Cas9, which indicates the suitability of our approach for genome-wide functional screenings.

MeSH terms

  • Clustered Regularly Interspaced Short Palindromic Repeats*
  • Codon, Nonsense
  • Codon, Terminator / genetics*
  • Gene Expression Regulation
  • Gene Silencing*
  • Gene Targeting / methods
  • Genetic Vectors
  • HEK293 Cells
  • Humans
  • Plasmids


  • Codon, Nonsense
  • Codon, Terminator