doi: 10.1038/nmeth.4324.
Epub 2017 Jun 5.
Differential analysis of RNA-seq incorporating quantification uncertainty
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- PMID: 28581496
- DOI: 10.1038/nmeth.4324
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Differential analysis of RNA-seq incorporating quantification uncertainty
Nat Methods.
2017 Jul.
Free article
Abstract
We describe sleuth (http://pachterlab.github.io/sleuth), a method for the differential analysis of gene expression data that utilizes bootstrapping in conjunction with response error linear modeling to decouple biological variance from inferential variance. sleuth is implemented in an interactive shiny app that utilizes kallisto quantifications and bootstraps for fast and accurate analysis of data from RNA-seq experiments.
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