Differential analysis of RNA-seq incorporating quantification uncertainty

Nat Methods. 2017 Jul;14(7):687-690. doi: 10.1038/nmeth.4324. Epub 2017 Jun 5.


We describe sleuth (http://pachterlab.github.io/sleuth), a method for the differential analysis of gene expression data that utilizes bootstrapping in conjunction with response error linear modeling to decouple biological variance from inferential variance. sleuth is implemented in an interactive shiny app that utilizes kallisto quantifications and bootstraps for fast and accurate analysis of data from RNA-seq experiments.

MeSH terms

  • Base Sequence
  • Computer Simulation*
  • Gene Expression / physiology*
  • Models, Biological
  • RNA / genetics*
  • Software*


  • RNA