An Enzyme-Induced Novel Biosensor for the Sensitive Electrochemical Determination of Isoniazid

Biosensors (Basel). 2017 Jun 5;7(2):21. doi: 10.3390/bios7020021.


In this present work, a glassy carbon electrode (GCE) was modified primarily with multiwalled carbon nanotubes (MWCNTs) and a composite of MWCNTs and titanium oxide nanoparticles (TiO₂NPs). The enzyme horseradish peroxidase (HRP) was immobilized to enhance the sensing ability of GCE. The proposed biosensor was used for the sensitive determination of isoniazid (INZ) in various pharmaceutical samples. The electrochemical behaviour of the developed MWCNT-TiO₂NPs-HRP-GCE biosensor was studied by using cyclic voltammetry (CV) and differential pulse voltammetric (DPV) techniques. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetry (TGA) and transmission electron microscopy (TEM) techniques were used to characterize the developed sensor. Phosphate buffer solution (PBS) with pH 7 was used as supporting electrolyte in the present investigation. The cyclic voltammetric results revealed that the increment of anodic peak currents for the enzyme-induced sensor was almost 8-fold greater than that of a bare GCE. The DPV technique exhibited good limit of detection and limit of quantification values, viz., 0.0335 μM and 0.1118 μM, respectively. Moreover, the developed sensor showed long-lasting stability and repeatability without any interferents. This strongly indicates that the fabricated sensor shows outstanding electrochemical performance towards INZ, with excellent selectivity and sensitivity. The developed sensor was successfully applied to pharmaceutical samples and gave good percentages of recoveries.

Keywords: cyclic voltammetry; differential pulse voltammetry; enzyme-based biosensor; isoniazid; pharmaceutical samples.

MeSH terms

  • Biosensing Techniques / methods*
  • Electrochemical Techniques
  • Horseradish Peroxidase / chemistry
  • Isoniazid / isolation & purification*
  • Limit of Detection
  • Metal Nanoparticles / chemistry*
  • Nanotubes, Carbon / chemistry
  • Spectroscopy, Fourier Transform Infrared
  • Titanium / chemistry


  • Nanotubes, Carbon
  • titanium dioxide
  • Titanium
  • Horseradish Peroxidase
  • Isoniazid