Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
, 58 (9), 1367-1372

Functional Optical Imaging of Primary Human Tumor Organoids: Development of a Personalized Drug Screen

Affiliations
Review

Functional Optical Imaging of Primary Human Tumor Organoids: Development of a Personalized Drug Screen

Alex J Walsh et al. J Nucl Med.

Abstract

Primary tumor organoids are a robust model of individual human cancers and present a unique platform for patient-specific drug testing. Optical imaging is uniquely suited to assess organoid function and behavior because of its subcellular resolution, penetration depth through the entire organoid, and functional endpoints. Specifically, optical metabolic imaging (OMI) is highly sensitive to drug response in organoids, and OMI in tumor organoids correlates with primary tumor drug response. Therefore, an OMI organoid drug screen could enable accurate testing of drug response for individualized cancer treatment. The objective of this perspective is to introduce OMI and tumor organoids to a general audience in order to foster the adoption of these techniques in diverse clinical and laboratory settings.

Keywords: 3D culture; cancer drug screens; fluorescence imaging; multiphoton microscopy; optical; primary human spheroids.

Similar articles

See all similar articles

Cited by 7 articles

See all "Cited by" articles

MeSH terms

LinkOut - more resources

Feedback