Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression

Sci Rep. 2017 Jun 6;7(1):2831. doi: 10.1038/s41598-017-03053-9.


Black yeast, Aureobasidium pullulans is extracellularly produced β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. In this study, using Glycine max cv. Kurosengoku (Kurosengoku soybeans), the production of β-glucan through fermentation of A. pullulans was evaluated, and the effects of A. pullulans cultured fluid (AP-CF) containing β-glucan made with Kurosengoku soybeans (kAP-CF) on a human monocyte derived cell line, Mono Mac 6 cells were investigated. Concentration of β-glucan in kAP-CF reached the same level as normal AP-CF. An anti-angiogenic protein, Thrombospondin-1 (THBS1) was effectively induced after the stimulation with kAP-CF for comparison with AP-CF. The THBS1 is also induced after stimulation with hot water extract of Kurosengoku soybeans (KS-E), while the combined stimulation of β-glucan with KS-E more effectively induced THBS1 than that with KS-E alone. These results suggest effects of A. pullulans-produced β-glucan on the enhancement of Kurosengoku soybean-induced THBS1 expression.

MeSH terms

  • Ascomycota / metabolism*
  • Cell Line
  • Fermentation
  • Gene Expression Regulation / drug effects*
  • Glycine max / chemistry*
  • Humans
  • Macrophage-1 Antigen / metabolism
  • Monocytes / drug effects
  • Monocytes / metabolism
  • Plant Extracts / pharmacology*
  • RNA, Messenger / genetics
  • Thrombospondin 1 / genetics*
  • beta-Glucans / pharmacology*


  • Macrophage-1 Antigen
  • Plant Extracts
  • RNA, Messenger
  • Thrombospondin 1
  • beta-Glucans