PHLDA3 impedes somatic cell reprogramming by activating Akt-GSK3β pathway

Sci Rep. 2017 Jun 6;7(1):2832. doi: 10.1038/s41598-017-02982-9.


Reprogramming of adult somatic cells into induced pluripotent stem cells holds great promise in clinical therapy. Increasing evidences have shown that p53 and its target genes play important roles in somatic cell reprogramming. In this study, we report that PHLDA3, a p53 target gene, functions as a blockage of iPSCs generation by activating the Akt-GSK3β pathway. Furthermore, PHLDA3 is found to be transcriptionally regulated by Oct4. These findings reveal that PHLDA3 acts as a new member of the regulatory network of somatic cell reprogramming.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cellular Reprogramming*
  • Gene Expression
  • Gene Expression Regulation, Developmental
  • Glycogen Synthase Kinase 3 beta / metabolism*
  • Humans
  • Induced Pluripotent Stem Cells / metabolism
  • Mice
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Octamer Transcription Factor-3 / metabolism
  • Proto-Oncogene Proteins c-akt / metabolism*
  • Transcription, Genetic


  • Nuclear Proteins
  • Octamer Transcription Factor-3
  • TSSC3 protein
  • Glycogen Synthase Kinase 3 beta
  • Proto-Oncogene Proteins c-akt