Follicular Dendritic Cell Isolation and Loading of Immune Complexes

Methods Mol Biol. 2017;1623:105-112. doi: 10.1007/978-1-4939-7095-7_9.


Follicular dendritic cells (FDCs) are stromal cells that are centrally located within B cell follicles of lymph nodes and other lymphoid organs such as the spleen. Due to their relative low abundance and difficulty to isolate, FDCs are still largely an enigma. Here we describe how to isolate FDCs for ex vivo cell culture, sorting by flow cytometry and how to load them in vivo or in vitro with immune complexes.

Keywords: Cell culture; Cell isolation; FACS; Follicular dendritic cell; Immune complex.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antigen-Antibody Complex / immunology*
  • Antigen-Antibody Complex / metabolism*
  • Biomarkers
  • Dendritic Cells, Follicular / immunology*
  • Dendritic Cells, Follicular / metabolism*
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Immunophenotyping
  • Lymph Nodes / cytology
  • Lymph Nodes / immunology
  • Lymph Nodes / metabolism
  • Mice
  • Spleen / cytology
  • Spleen / immunology
  • Spleen / metabolism


  • Antigen-Antibody Complex
  • Biomarkers