Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Jul;23(7):1133-1143.
doi: 10.1097/MIB.0000000000001155.

Supplementation of Low- And High-fat Diets With Fermentable Fiber Exacerbates Severity of DSS-induced Acute Colitis

Affiliations
Free PMC article

Supplementation of Low- And High-fat Diets With Fermentable Fiber Exacerbates Severity of DSS-induced Acute Colitis

Jennifer P Miles et al. Inflamm Bowel Dis. .
Free PMC article

Abstract

Background: Lack of dietary fiber has been suggested to increase the risk of developing various chronic inflammatory diseases, whereas supplementation of diets with fiber might offer an array of health-promoting benefits. Consistent with this theme, we recently reported that in mice, compositionally defined diets that are made with purified ingredients and lack fermentable fiber promote low-grade inflammation and metabolic syndrome, both of which could be ameliorated by supplementation of such diets with the fermentable fiber inulin.

Methods: Herein, we examined if, relative to a grain-based mouse diet (chow), compositionally defined diet consumption would impact development of intestinal inflammation induced by dextran sulfate sodium (DSS) and moreover, whether DSS-induced colitis might also be attenuated by diets supplemented with inulin.

Results: Analogous to their promotion of low-grade inflammation, compositionally defined diet of high- and low-fat content with cellulose increased the severity of DSS-induced colitis relative to chow. However, in contrast to the case of low-grade inflammation, addition of inulin, but not the insoluble fiber cellulose, further exacerbated the severity of colitis and its associated clinical manifestations (weight loss and bleeding) in both low- and high-fat diets.

Conclusions: While inulin, and perhaps other fermentable fibers, can ameliorate low-grade inflammation and associated metabolic disease, it also has the potential to exacerbate disease severity in response to inducers of acute colitis.

Conflict of interest statement

COMPETING INTEREST

None.

Figures

Figure 1
Figure 1. Compositionally-defined diet results in severe disease in DSS-treated mice
C57Bl/6 male mice were maintained on the specified diet for 7 days and subsequently treated with DSS 2.5% for 6 days. A. Body weight over time. B. Caecum/colon gross morphology. C. Spleen weight. D. Caecum weight. E. Colon length. Data are represented as means ± SEM of N=3–6 mice per group. Significance was determined by Student t test. *P < 0.05.
Figure 2
Figure 2. Fermentable fiber inulin does not protect against DSS challenge in purified diet-fed mice
C57Bl/6 male mice were maintained on the specified diet for 7 days and subsequently treated with DSS 2.5% for 7 days. A. Body weight over time. B. Colon length. C. Caecum weight. D. Spleen weight. Data are represented as means ± SEM of N=5 mice per group. Significance was determined by Student t test. *P < 0.05.
Figure 3
Figure 3. Fermentable fiber inulin worsens intestinal inflammation in DSS-treated mice under purified diet
C57Bl/6 male mice were maintained on the specified diet for 7 days and subsequently treated with DSS 2.5% for 7 days. A–C. Fecal lipocalin-2 levels at day 0 (A), day 3 (B) and day 7 (C). D. Development of rectal bleeding over time. E. Colonic histopathological scoring. F. H&E staining of colonic tissues. Data are represented as means ± SEM of N=5 mice per group. Significance was determined by Student t test. *P < 0.05.
Figure 4
Figure 4. Fermentable fiber inulin does not protect against DSS challenge in high-fat diet-fed mice
C57Bl/6 male mice were maintained on the specified diet for 7 days and subsequently treated with DSS 2.5% for 6 days. A. Body weight over time. B. Colon length. C. Caecum weight. D. Spleen weight. E. Colon weight. Data are represented as means ± SEM of N=3–5 mice per group. Significance was determined by Student t test. *P < 0.05.
Figure 5
Figure 5. Absence of IL-22 does not impact inulin’s exacerbation of DSS colitis
IL-22KO male mice were maintained on the specified diet for 7 days and subsequently treated with DSS 2.5% for 5 days. A. Body weight over time. B. Colon length. C. Colon weight. D. Feces supernatant gross picture. Data are represented as means ± SEM of N=4–5 mice per group. Significance was determined by Student t test. *P < 0.05.
Figure 6
Figure 6. Fermentable fiber inulin dampened basal intestinal inflammation but does not protect against DSS challenge in chow-fed mice
C57Bl/6 male mice were maintained on the specified diet for 7 days and subsequently treated with DSS 2.5% for 7 days. A. Body weight over time. B. Colon weight. C. Colon length. D. Caecum weight. E–F. Fecal lipocalin-2 levels at day 0 (E, post diet/pre DSS) and day 3 (F, post diet/post DSS). Data are represented as means ± SEM of N=5 mice per group. Significance was determined by Student t test. *P < 0.05.

Similar articles

See all similar articles

Cited by 17 articles

See all "Cited by" articles

Publication types

Feedback