A cellular gene encodes scrapie PrP 27-30 protein

Cell. 1985 Apr;40(4):735-46. doi: 10.1016/0092-8674(85)90333-2.


A clone encoding PrP 27-30, the major protein in purified preparations of scrapie agent, was selected from a scrapie-infected hamster brain cDNA library by oligonucleotide probes corresponding to the N terminus of the protein. Southern blotting with PrP cDNA revealed a single gene with the same restriction patterns in normal and scrapie-infected brain DNA. A single PrP-related gene was also detected in murine and human DNA. PrP-related mRNA was found at similar levels in normal and scrapie-infected hamster brain, as well as in many other normal tissues. Using antisera against PrP 27-30, a PrP-related protein was detected in crude extracts of infected brain and to a lesser extent in extracts of normal brain. Proteinase K digestion yielded PrP 27-30 in infected brain extract, but completely degraded the PrP-related protein in normal brain extract. No PrP-related nucleic acids were found in purified preparations of scrapie prions, indicating that PrP 27-30 is not encoded by a nucleic acid carried within the infectious particles.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain Chemistry
  • Cloning, Molecular
  • Cricetinae
  • DNA / analysis*
  • Humans
  • Mice
  • Nucleic Acid Hybridization
  • Nucleic Acids / analysis
  • Poly A / genetics
  • Poly A / isolation & purification
  • PrP 27-30 Protein
  • Prions
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Scrapie / genetics*
  • Sheep
  • Viral Proteins / analysis*
  • Viral Proteins / genetics*


  • Nucleic Acids
  • Prions
  • RNA, Messenger
  • Viral Proteins
  • PrP 27-30 Protein
  • Poly A
  • DNA

Associated data

  • GENBANK/K02234