Pre-banking microbial contamination of donor conjunctiva and storage medium for penetrating keratoplasty

Jpn J Ophthalmol. 2017 Sep;61(5):369-377. doi: 10.1007/s10384-017-0521-1. Epub 2017 Jun 8.


Purpose: The aims of this study were to investigate the incidence of positive donor tissue cultures before transfer to preservation medium (Optisol™-GS) for penetrating keratoplasty, to verify the efficacy of antibiotics contained in Optisol™-GS by examining the drug susceptibility and to assess the relationship between the results of our microbial assessments as well as donor factors and the incidence of contamination.

Methods: We conducted a retrospective, cross-sectional study using Juntendo Eye Bank records for all corneal transplantations. Two hundred donor conjunctiva harvestings and storage medium (EP-II®) cultures were performed between July 2008 and June 2011. We analyzed the associations between donor factors (age, gender, history of cataract surgery, death-to-preservation interval, cause of death) and contamination rates using multivariate analysis by the generalized estimating equation model.

Results: We obtained positive bacterial cultures from 154 of the 200 eyes (77.0%). The isolated bacteria were indigenous, such as coagulase-negative Staphylococci, Corynebacterium sp., and methicillin-resistant Staphylococcus aureus (MRSA). There was significant resistance to levofloxacin (18 eyes, 9.0%) and gentamicin (12 eyes, 6.0%), and no vancomycin-resistant bacteria were detected. The donor factors did not correlate with the prevalence of bacterial contamination in our criteria.

Conclusions: Pre-banking microbial assessment allows for microbial detection, bacterial susceptibility and resistance testing. This is useful for developing preservation mediums containing effective spectrum antibiotic agents for high quality control of corneal banking.

Keywords: Bacterial contamination; Corneoscleral rim; Donor conjunctiva; Eye banking; Preservation medium.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Bacteria / isolation & purification*
  • Chondroitin Sulfates / pharmacology*
  • Complex Mixtures / pharmacology
  • Conjunctiva / microbiology*
  • Cross-Sectional Studies
  • Culture Media, Serum-Free
  • Dextrans / pharmacology*
  • Eye Banks*
  • Eye Infections, Bacterial / diagnosis*
  • Eye Infections, Bacterial / microbiology
  • Female
  • Gentamicins / pharmacology*
  • Humans
  • Keratoplasty, Penetrating*
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Organ Preservation*
  • Retrospective Studies
  • Tissue Donors


  • Complex Mixtures
  • Culture Media, Serum-Free
  • Dextrans
  • Gentamicins
  • Chondroitin Sulfates
  • Optisol