Pseudoxanthomonas putridarboris sp. nov. isolated from rotten tree

Int J Syst Evol Microbiol. 2017 Jun;67(6):1807-1812. doi: 10.1099/ijsem.0.001867. Epub 2017 Jun 9.


A Gram-staining-negative, rod-shaped, motile bacterium, designated WD12T, was isolated from a rotten tree at Chungbuk National University, South Korea. WD12T grew optimally at 30-37 °C and pH 7.0-7.5 and could assimilate arbutin and potassium-5-ketogluconate. The major cellular fatty acid were iso-C16 : 0, C16 : 0, cyclo C17 : 0, iso-C15 : 0, summed features 3 (comprising C16 : 1ω7c/iso-C15 : 0 2-OH) and anteiso-C15 : 0. The major polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was ubiquinone-8 (Q-8). The G+C content of the genomic DNA was 69.1 %. The results of phylogenetic and comparative analysis based on the 16S rRNA gene sequence indicated that WD12T formed a tight phylogenetic lineage with Pseudoxanthomonas mexicana AMX 26BT and Pseudoxanthomonas japonensis 12-3T of the the genus Pseudoxanthomonas in the family Xanthomonadaceae. Sequence similarity to other members of the genus Pseudoxanthomonasranged from 98.6 % (P. mexicana AMX 26BT) to 95.1 % (Pseudoxanthomonas taiwanensis CB-226T). DNA-DNA relatedness between WD12T and eight type strains of species of the genus Pseudoxanthomonasshowing more than 97 % 16S rRNA sequence similarity were 6±0-26±1 %. On the basis of the evidence from this polyphasic study, WD12T represents a novel species of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas putridarboris sp. nov. is proposed. The type strain is WD12T (=KACC 15045T=LMG 25968T).

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Nucleic Acid Hybridization
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Sequence Analysis, DNA
  • Ubiquinone / chemistry
  • Wood / microbiology*
  • Xanthomonadaceae / classification*
  • Xanthomonadaceae / genetics
  • Xanthomonadaceae / isolation & purification


  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • ubiquinone 8