Evaluating a novel oxygenating therapeutic for its potential use in the advancement of wound healing

Toxicol In Vitro. 2017 Sep:43:62-68. doi: 10.1016/j.tiv.2017.06.005. Epub 2017 Jun 7.


Non-gaseous oxygen therapeutics are emerging technologies in regenerative medicine that aim to sidestep the undesirable effects seen in traditional oxygen therapies, while enhancing tissue and wound regeneration. Using a novel oxygenating therapeutic (Ox66™) several in vitro models including fibroblast and keratinocyte monocultures were evaluated for potential drug toxicity, the ability of cells to recover after chemical injury, and cell migration after scratch assay. It was determined that in both cell lines, there was no significant cytotoxicity found after independent treatment with Ox66™. Similarly, after DMSO-induced chemical injury, the health parameters of cells treated with Ox66™ were improved when compared to their untreated counterparts. Particles were also characterized using scanning electron microscopy and electron dispersive spectroscopy both individually and in conjunction with fibroblast growth. The data in this study showed that the novel wound healing therapeutic has potential in advancing the treatment of various types of acute and chronic wounds.

Keywords: Fibroblast; Keratinocyte; Oxygen; Scanning electron microscopy; Topical oxygen therapy; Wound healing.

MeSH terms

  • Cell Survival / drug effects
  • Cells, Cultured
  • Fibroblasts / drug effects*
  • Humans
  • Keratinocytes / drug effects*
  • Oxygen / therapeutic use*
  • Wound Healing / drug effects*


  • Oxygen