Sequential IGF-1 and BMP-6 releasing chitosan/alginate/PLGA hybrid scaffolds for periodontal regeneration

Int J Biol Macromol. 2017 Nov;104(Pt A):232-241. doi: 10.1016/j.ijbiomac.2017.06.029. Epub 2017 Jun 7.


The goal of periodontal tissue engineering is to repair or regenerate the destructed or lost periodontium by improving functions of cells in the remaining tissue. For continuty of cell growth process, two group of growth factors, i.e. competence factors and progression factors, are needed to act together. However, the short biological half-life of these factors limits their effects on cells and their clinical efficacy. The purpose of this study is to develop different microparticles-loaded chitosan carriers/scaffolds for controlled and sequential delivery of a competence factor, insulin-like growth factor (IGF-1), and progression factor, bone morphogenetic factor-6 (BMP-6). Alginate and poly (lactic-co-glycolic acid) (PLGA) microparticles provided release of IGF-1 and BMP-6 for early short period and for long period, respectively. The cell culture studies showed that, chitosan/alginate/PLGA hybrid scaffolds induced proliferation and osteoblastic differentiation of cementoblasts when compared with IGF-1 and BMP-6 free chitosan scaffold.

Keywords: BMP-6; Cementoblasts; Chitosan; IGF-1; Periodontal regeneration; Scaffold.

MeSH terms

  • Alginates / chemistry*
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / pharmacology
  • Bone Morphogenetic Protein 6 / metabolism*
  • Cell Proliferation / drug effects
  • Chitosan / chemistry*
  • Gene Expression Regulation / drug effects
  • Glucuronic Acid / chemistry
  • Hexuronic Acids / chemistry
  • Insulin-Like Growth Factor I / metabolism*
  • Lactic Acid / chemistry*
  • Microspheres
  • Minerals / metabolism
  • Periodontium / drug effects*
  • Periodontium / physiology
  • Polyglycolic Acid / chemistry*
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Regeneration / drug effects
  • Tissue Scaffolds / chemistry*


  • Alginates
  • Biocompatible Materials
  • Bone Morphogenetic Protein 6
  • Hexuronic Acids
  • Minerals
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • Insulin-Like Growth Factor I
  • Glucuronic Acid
  • Chitosan