CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells

Stem Cell Reports. 2017 Jul 11;9(1):355-365. doi: 10.1016/j.stemcr.2017.05.010. Epub 2017 Jun 8.


Human pluripotent stem cells (PSCs) provide an unlimited cell source for cell therapies and disease modeling. Despite their enormous power, technical aspects have hampered reproducibility. Here, we describe a modification of PSC workflows that eliminates a major variable for nearly all PSC experiments: the quality and quantity of the PSC starting material. Most labs continually passage PSCs and use small quantities after expansion, but the "just-in-time" nature of these experiments means that quality control rarely happens before use. Lack of quality control could compromise PSC quality, sterility, and genetic integrity, which creates a variable that might affect results. This method, called CryoPause, banks PSCs as single-use, cryopreserved vials that can be thawed and immediately used in experiments. Each CryoPause bank provides a consistent source of PSCs that can be pre-validated before use to reduce the possibility that high levels of spontaneous differentiation, contamination, or genetic integrity will compromise an experiment.

Keywords: cell banking; cell therapy; cryopreservation; directed differentiation; disease modeling; human pluripotent stem cell; quality control.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Specimen Banks
  • Cell Differentiation
  • Cell Line
  • Cell- and Tissue-Based Therapy
  • Cryopreservation / methods*
  • Gene Editing
  • Humans
  • Mice
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / metabolism
  • Pluripotent Stem Cells / transplantation