Site-specific incorporation of phosphotyrosine using an expanded genetic code

Nat Chem Biol. 2017 Aug;13(8):842-844. doi: 10.1038/nchembio.2406. Epub 2017 Jun 12.


Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is convertible to native phosphotyrosine. We demonstrate its general compatibility with proteins of various sizes, phosphotyrosine sites and functions, and reveal a possible role of tyrosine phosphorylation in negative regulation of ubiquitination.

MeSH terms

  • Animals
  • Genetic Code / genetics*
  • Phosphorylation
  • Phosphotyrosine / genetics*
  • Phosphotyrosine / metabolism*
  • Proteins / chemistry
  • Proteins / genetics*
  • Proteins / metabolism*
  • Tyrosine / metabolism


  • Proteins
  • Phosphotyrosine
  • Tyrosine

Associated data

  • PubChem-Substance/336297853