Polysaccharides with immunomodulating activity from roots of Gentiana crassicaulis

Carbohydr Polym. 2017 Sep 15:172:306-314. doi: 10.1016/j.carbpol.2017.04.049. Epub 2017 Apr 23.

Abstract

Two polysaccharides, GCP-I-I and GCP-II-I, were obtained from 100°C water extracts of Gentiana crassicaulis roots by DEAE anion exchange chromatography and gel filtration. The results from methanolysis, methylation, FT-IR and NMR, indicated that these two fractions are typical pectic polysaccharides, with HG and RG-I regions and AG-I/AG-II side chains, and some of the galacturonic acid units of fraction GCP-I-I were methyl esterified. Fractions GCP-I-I and GCP-II-I, both exhibited potent complement fixation, and fraction GCP-I-I was more potent than positive control BPII. The higher complement fixation activity obtained in fraction GCP-I-I may be due to the higher Mw and/or higher amount of AG-II present in fraction GCP-I-I than fraction GCP-II-I. The polysaccharides from G. crassicaulis could be used as a potential natural immunomodulator.

Keywords: Complement system; Gentiana crassicaulis; Polysaccharides; Purification; Trifluoroacetic acid (PubChem CID: 6422); d-Fructose (PubChem CID: 5984); d-Galactose (PubChem CID: 6036); d-Galacturonic acid (PubChem CID: 439215); d-Glucose (PubChem CID: 5793); d-Glucuronic acid (PubChem CID: 94715); d-Mannose (PubChem CID: 18950); d-Xylose (PubChem CID: 135191); l-Arabinose (PubChem CID: 439195); l-Rhamnose (PubChem CID: 25310).

MeSH terms

  • Animals
  • Chromatography, Gel
  • Complement Fixation Tests
  • Erythrocytes / drug effects*
  • Gentiana / chemistry*
  • Plant Roots / chemistry*
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Sheep
  • Spectroscopy, Fourier Transform Infrared

Substances

  • Polysaccharides